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What is MALBAC?

Introduction
MALBACTM (Multiple Annealing and Looping-based Amplification Cycles) is a patented whole genome amplification (WGA) technology developed at Harvard University. The special design enables a quasi-linear pre-amplification of the whole genome at the single cell level followed by an exponential amplification, yielding 2-4μg amplification produc suitable for downstream analysis using next-generation sequencing, microarray, or real-time qPCR, etc.
Features
• Extreme low input (single cell or pg level gDNA)

• Quasi-linear whole genome amplification with the least amplification bias

• High sequencing coverage and uniformity

• Low allele dropout rate

• High reproducible WGS results

• Single tube reaction with minimal sample loss

• Easy sample prep with 3 steps in 4 hours

• Suitable for CNV/SNV analysis of stem cells, CTCs, microbes, or plant cells, etc.
MALBACTM WGA Technology
Comparison of Key Parameters of MALBACTM WGA Technology With Other Commercial Platforms
Yikon’s MALBACTM single-cell whole genome amplification technology has low coefficient of variation, allele dropout rate and unmappable rate.

Important Discoveries Using MALBACTM WGA Technology
The comprehensive analyses of human oocyte and sperm meiotic recombination were performed using MALBACTM single cell sequencing. By sequencing first, second polar body (PB1 and PB2), human oocyte pronucleus and sperm, the recombination maps were generated for human germ cells at high resolution for the first time (Figure 2).

These studies also established a standard procedure for MALBACTM-based preimplantation genetic screening (PGS) and preimplantation genetic diagnosis (PGD) for in vitro-fertilized embryos.
MALBACTM single-cell whole genome sequencing was applied to examine the copy number variation (CNV) of circulating tumor cells (CTCs) (Figure 3).
Results showed that CNV patterns of CTCs were reproducible within a patient and even among different patients of the same cancer type.
Moreover, CNV patterns of CTCs are similar to those of the metastatic but not the primary tumors.
MAGBIO GENOMICS
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