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ULTRAPURE LIGASES
Product Name Part No. Unit Size Concentration Price Notes
T3 DNA Ligase L6010L 900,000 U 3,000,000 U/ml 534,000 Works in high ionic strength – to 1.0 M NaCl.
T7 DNA Ligase L6020L 900,000 U 3,000,000 U/ml 534,000 1000 fold higher activity on sticky ends than blunt ends.
T4 DNA Ligase (Rapid) L6030-HC-L 240,000 U 600,000 U/ml 755,000 “Ultrapure ligase” referenced in Nature Methods (Quail, 1 December 2008).
T4 DNA Ligase L6030-LC-L 150,000 U 120,000 U/ml 534,000 Low concentration overnight ligation format.
T4 RNA Ligase 1 L6050L 10,000 U 20,000 U/ml 534,000 Single-stranded RNA ligase. Also joins single-stranded DNA molecules.
Taq DNA Ligase L6060L 3,100 U 40,000 U/ml 534,000 Thermostable DNA ligase which efficiently seals nicks and discriminates against mismatch ligation.
T4 RNA Ligase 2 L6080L 4,500 U 30,000 U/ml 534,000 Ligates nicks on double stranded DNA and from the 3′ OH of RNA to the 5′ phosphate of DNA in double stranded structures.
E. coli DNA Ligase L6090L 2,500 U 10,000 U/ml 534,000 Efficiently ligates DNA at nicks and cohesive termini. Blunt-ended termini can be joined in the presence of PEG.

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