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High Efficiency CRISPR Editing in Primary and Stem cells
이노라이프텍 조회수:1713 115.161.97.118
2019-09-19 10:44:54

High Efficiency CRISPR Editing in Primary and Stem cells

CRISPR mediated gene editing in stem and primary cells is more challenging than in established cell
lines. Increased exonuclease activity and immuno-stimulatory responses to exogenous nucleic acids may
contribute to reduced RNA guide activity and consequently lower editing rates.
Synthego’s chemically modified synthetic sgRNA can significantly mitigate these effects, producing
consistent editing rates of 90% or more in primary and stem cells.

 

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