The Protocols Pack (Lipofection, Nucleofection, Electroporation, iPS cells ) describes how to transfect cultured cells with ribonucleoprotein (RNP) complexes that consist of purified Cas9 nuclease duplexed with synthetic guide RNA (gRNA; synthetic sgRNA or annealed crRNA and tracrRNA). Combined with high-quality synthetic guide RNA, you’ll be able to achieve unparalleled consistency between your CRISPR experimental replicates.
1. Lipofection of Cas9/synthetic RNA ribonucleoprotein (RNP) complexes for CRISPR/Cas9 genome editing (Thermo CRISPRMAX™ Kit)
2. Electroporation of Cas9/Synthetic RNA Ribonucleoprotein (RNP) Complexes for CRISPR/Cas9 Genome Editing (Thermo Neon™ System)
3. Nucleofection (electroporation) of Cas9/synthetic RNA ribonucleoprotein (RNP) complexes for CRISPR/Cas9 genome editing (Lonza Nucleofection™ System)
4. Transfection of iPS cells derived from human fibroblasts with chemically modified synthetic guide RNA-Cas9 ribonucleoprotein (RNP) complexes using Lonza Nucleofection™